HubSpot TV – Gobble Up Inbound Marketing (Thanksgiving Episode)

first_img DealNewshas a free Black Friday app that lets you sort deals by “Early Bird,””In Store Only,” and “Limited Availability,” as well as see photos ofthe sale items. : Produce different kinds of to enhance the visibility of your company on Google search results. Everyone is welcome to come in at 4pm to the office for the live audience. Black Friday and Social Media How to interact on Twitter: @ Gizmodo’s #blackfriday feed has the latest posts about the best electronic deals to be had. @ “A whopping 91% percent ofcompanies also admitted to using at least one social media tool, asignificant increase over 77% of respondents in 2008.” Inbound Marketing Kit “Originallywritten in March of this year the update makes sense since the 8 monthsor so that have passed since the first take is like a lifetime in thesocial media world. In fact, the real story here may be that the LATimes hasn’t taken a ‘set it and forget it’ approach to their socialmedia guidelines so please take note everybody.” mvolpe . No show this Friday (Thankgiving Holiday) Click here at 4:00pm ET today to watch the show live. “Forty-five percent of respondents surveyed maintained ablog at their company in 2009 (a slight jump over the 39% in 2008 and19% in 2007 who blogged). Of course, the same growth didn’t extendacross all social platforms.” Learning From Leaders: The Inc. 500 and Social Media Usage Mike has a moustache for Movember! support him at NOTE FROM THE PRODUCERS: : Create social media guidelines for your company that GUIDE your employees but don’t stop them from creating content. Have you ever caught the live show? We broadcast in real-time every Friday at Where is your traffic coming from? inbound marketing kit Staples, Macy’s and Walmart are “leaking” deals on Twitter with The fastest growing companies are using social media & inbound marketing, shouldn’t you? – Connect with your customers in every way possible. (Episode Length: 19 minutes, 15 seconds) karenrubin Conversion Rate vs. Number of Sales AlisonSemancik asks on inboundmarketing.comcenter_img Episode #68 – November 25, 2009 LA Times Social Media Guidelines Lead to Much More Marketing Takeaway Google Adds a Left Navigation? Google Testing Permanent Sidebar “Thenew interface uses a left-hand pane to display search options-butinstead of being off by default, with a link to turn on the pane, thenew search options will be on permanently, and Google will lose the topblue bar over its results.” Marketing Takeaway The Inc 500 is Catching On to Inbound Marketing Subscribe in iTunes: MyBlack Friday Deals…uses the mobile photo slide show app Whrrl toallow bloggers to take pictures of the best deals they can find instores and upload them to the site to be voted on by deal seekers. Originally published Dec 4, 2009 3:30:00 PM, updated July 04 2013 : I just recently made a change to our “buy now” page – thepage where visitors can choose the software package that works best forthem and purchase. In the past, we only had 3 options to choose from: 1user, 3 user and 5 user. Our company recently decided to add a 10 userpackage to this page. Since I added it one week ago, sales have droppedsignificantly. I can only conclude that the sales drop is from thisaddition, but I’m not sure. Learn more about inbound marketing and how to combine blogging, SEO and social media for results. Download our Forum Fodder “The Times isto be, above all else, a principled news organization. In deed and inappearance, journalists must keep themselves – and The Times – abovereproach.” in your tweet. Google Website Optimize, HubSpot Analytics HOW TO: Use Social Media to Find Black Friday Deals Times updates social media guidelines always Don’t forget to share this post! AddThis Sharing ButtonsShare to TwitterTwitterShare to FacebookFacebookShare to Email AppEmail AppShare to LinkedInLinkedInShare to MessengerMessengerShare to SlackSlack Closing Intro LA Times Refines Social Media Guidelines “Thestudy found that 75% of companies who participated in the survey werevery familiar with social media in 2009, a dramatic increase over the57% of respondents familiar with some type of social networking in2008.” Marketing Tip of the Week Marketing Takeaway: : Your New Year’s Resolution should be to try something new (and we don’t mean just on the table!) Marketing Takeawaylast_img read more

Pinterest FAQs, Browser Bookmarks, and More in HubSpot Content This Week

first_img Originally published Feb 9, 2014 8:00:00 AM, updated February 01 2017 Topics: Pinterest Marketing Here’s a question: How do you become a better inbound marketer?  Well, for starters, there’s experience. By showing up everyday, putting in the work, and learning from those around you, you’ll naturally get better at what you do. Practice, after all, makes perfect.But for those marketers who have a healthy obsession with self-improvement and want to become inbound marketing experts, there’s a lot you can do to fast-track your progress. From checking out the best content on the web for inspiration, to leveraging tools that make you more efficient, to reading up on advice from industry experts, there are quite a few educational resources online that can help you become a better marketer.Not sure where you can find those resources? Just scroll down and you’ll find plenty featured on Inbound Hub this past week.20 Browser Bookmarks Every Marketer Should Install [SlideShare]Looking for some online inspiration for your marketing? You could do a simple search for what you’re interested in. You could also find some good stuff on Twitter, LinkedIn, Facebook, and the like. Or, you could simply check out these awesome marketing articles, tools, and other resources that we’ve collected and neatly packaged for you. Go ahead and bookmark ’em — you’ll never be at a loss for inspiration.Pinterest FAQs: Answers to 11 Questions You’ve Always Wanted to AskHere’s the thing: If you want to become a master of a particular tool or discipline — including Pinterest — asking questions is indispensable to helping you learn. In this new post, we’ve compiled (and answered) 11 of the most common questions we’ve heard about Pinterest to give you the down-low on best practices for the social platform.How to Brainstorm Content Ideas in a Fast, Easy, Visual WayIf you’re a marketer with 99 problems, there’s a good chance content creation is one of them. And for many marketers, it isn’t actually the writing or creating that’s the problem — it’s figuring out what the heck you should create! In this recent post, we present to you our Content Brainstorming Key: an interactive document that guides you through the content ideation process.How to Write Effective and Engaging Meta DescriptionsThe best part about this post? Its meta description is about writing meta descriptions … how meta! In the post from our Insiders section, we show you how to turn the unceremonious meta description into a compelling call-to-action that convinces searchers to click through to your content.”They Said Email Was Dying”: How BuzzFeed Revived the Email NewsletterWhen thinking of the websites you can look to for marketing advice and inspiration, BuzzFeed probably wouldn’t be the first site you think of. But guess what? When it comes to email newsletters, BuzzFeed is one of the best. Need proof? The site offers 14 different newsletters to cater to different interests, and all of its emails are mobile-optimized. Hit the jump to learn more!How Salesforce Reached $3B in Revenue by Improving Management ProductivityWish your sales organization was a lean, mean, money-making machine? Good news: Elay Cohen,’s former SVP of sales productivity, has some great advice for you. In his post, featured on Inbound Hub’s Sales section, Elay walks through three key steps to improving management productivity.Check Out This Insane Super Bowl Ad That Hardly Anybody SawI think the moral of this Dan Lyons story — which ran in our Opinion section — is this: No matter how small, local, or niche your business is, you can still make a splash with stellar content. Dan highlights a ridiculously action-packed Super Bowl ad for a personal injury lawyer that only aired in the Savannah, Georgia area. Words can’t really do it justice … but I promise you that “justice” is a major theme in this explosive TV spot.What was the most interesting thing you learned this week on Inbound Hub? What do you want to see more of? Leave your feedback in the comments! Don’t forget to share this post! AddThis Sharing ButtonsShare to TwitterTwitterShare to FacebookFacebookShare to Email AppEmail AppShare to LinkedInLinkedInShare to MessengerMessengerShare to SlackSlacklast_img read more

How to Get More People to Open Your Emails [Video]

first_img Originally published Jun 2, 2014 8:00:00 AM, updated February 01 2017 Topics: For email marketers, there are few things more nerve-wracking than the anticipation you get after hitting send. Unlike other types of marketing, you’ve only got one chance with an email send to get someone to open your email. You cross your fingers and hope. Will people open this email? Will they hit spam? Will they actually click on it? Pleaseohpleaseohplease go well. Wouldn’t it be nice to know beforehand if your email would get opened?Full disclosure: You won’t be able to predict the future. But you can stack the deck in your favor by following a few best practices for increasing email open rates. Even though open rates shouldn’t be the only metric you base your success on, it’s still important to optimize for opens — after all, people need to open your email to be able to click on it. So in the third part of our email marketing series, we’ll walk you through three essential tips to getting more people to open your emails.Want to read the transcript instead of watching the video above? Here you go:How to Get More People to Open Your Emails [Transcription]Raise your hand if you wish you had more email every day?Anybody? You? … Yeah, I didn’t think so. Let’s face it: Every email inbox in the world is completely cluttered. From those annoying LinkedIn notifications and the Twitter email that fires off every time someone new follows you, we’ve been completely inundated.So as a marketer, you’ve got a lot to compete with. When you’re going up against all of those emails, your email has to stand out.  And the best way to do that? Write a compelling subject line. Today I’m going to give you three tips to improve your subject lines so you can increase your engagement and improve your open rates. Tip #1: Optimize Your Sender Name and Preview TextLet’s just take a minute and look at your inbox. What do you see? In most email providers this a typical message layout. It has three elements:Sender nameSubject lineMessage previewThese are the three elements that your reader will see and that will most influence them to open your message. The key to optimizing your sender name is to make it something that is easily recognizable to your subscribers so they want to open it. For most companies, this mean your sender name includes your company name.But there’s lots of ways you can get creative here.We conducted a test to compare a generic “HubSpot” sender name to a personal name of someone from the marketing team. Our control generated a 0.73% CTR, and the personalized version generated a 0.96% CTR. With a confidence level of 99.9%, we had a clear winner. Our conclusion after conducting this A/B test was that emails sent by a real person are more likely to be clicked on than emails sent from a company name.On your next email send, try changing out your sender name from your company name to your personal name.The preview text is a little easier — it should be something that follows up to your subject line. Maybe it answers your subject line’s question, or just follows along with the same theme. For example, check out this awesome preview from BuzzFeed’s email: It made me laugh … and I opened the email.Don’t forget about your “From” field and preview text. These are just as important as the subject line.Tip #2: Personalize Your Subject LinesA recent MarketingSherpa case study showed that personalized email subject lines can increase your clickthrough rates by 17.36%. But, most companies underutilize personalization or completely leave it out all together. If you have the data to personalize your subject lines, then use it. You can get higher clickthrough rates by testing first name and full name personalizations in your subject lines. For example, if your email recipient has recently downloaded a piece of content, try personalizing the subject by thanking them and sharing a reminder link to that content. Include their first names or even add something about their specific location. You definitely don’t want to go overboard here. You can easily cross the line to creepy-company-who’s-stalking-their-customer mode, and no one want to go there. Adding these little personal touches shows that you care about them — not just getting their email address.Tip #3: Avoid SPAM TriggersEmail spammers rely heavily on certain words to boost their open rates. Because of this, email providers use spam filters to keep out emails using these words.As an email marketer, you need to know what these words are and avoid them. You need to avoid using words like “Cash,” “Quote,” and “Save.” That being said, spam filters look at more than just subject lines to determine if they will deliver your email. For example, “Free” is a traditionally spammy subject line word, but you would notice that we included it in our “101 Companies Rocking Social Media” subject line.We did a lot of background research to make sure this word wouldn’t work against us. Because we have a solid sender reputation, and we were sending out an email from a real person, including “free” in this line didn’t impact the email’s deliverability. Moral of the story here is to be wary of spam trigger words. Don’t avoid them completely, but use them in a way that is non-spammy. So, remember … personalize your subejct lines, test your preview text and sender name, and avoid using spam triggers. These little tweaks could help you get found, opened, and engaged with in your subscribers’ busy inboxes. Until next time, happy marketing. Share This Video With OthersClick to tweet: Improve your email marketing conversion rates with this free 5-part video series from @HubSpot: Email Subject Lines Don’t forget to share this post! AddThis Sharing ButtonsShare to TwitterTwitterShare to FacebookFacebookShare to Email AppEmail AppShare to LinkedInLinkedInShare to MessengerMessengerShare to SlackSlacklast_img read more

A Simple Guide to Web Traffic Terms

first_img Originally published Jul 15, 2014 8:00:00 AM, updated July 28 2017 Topics: Views, visits, visitors — it’s all the same, right? Not so fast, speed racer. If that’s your way of thinking, you could be misinterpreting your traffic data and making some ill-informed decisions.  Because lots of people tend to throw these various terms around all willy-nilly, I’ve compiled a nice little cheat sheet so you can truly understand what’s what, interpret your website analytics correctly, and make well-informed, data-backed decisions about your marketing. Before you read on, it’s important to note that various analytics tools may have slightly different definitions of the following terms. It’s best to consult your particular tool’s documentation to understand exactly how each is calculated.Pageviews/ViewsA pageview (in Google Analytics) or a view (in HubSpot) is counted when a page on your site is loaded by a browser. It is counted every time the respective tool’s tracking code is loaded. In other words, if a person were to view a page on your site and reload the page in their browser, that would count as two views. If a person viewed one page, viewed a second page on your website, and then returned to the original page, that would count as three views.Pageview data is great for understanding your site’s stickiness. Are they visiting one page and then bouncing? Or are they sticking around to check out more of your website’s content?Unique PageviewsA calculation you’ll find in Google Analytics, unique pageviews aggregates pageviews that are generated by the same user during the same session (see definition of session below). A unique pageview is the number of sessions during which that page was viewed one or more times. So if a person viewed the same page twice (or more times) during an individual session, unique pageviews would only count that pageview once.VisitsIn general, a visit is when someone reaches your website from a referring source outside your website domain. Usually, analytics tools only attribute one visit even if a person goes to multiple pages on your site while they’re there. In other words, a person can accrue multiple pageviews during one visit. This is why pageview counts are generally larger than visit counts.In HubSpot’s software specifically, if a person were to visit your website, click a link that took them to another website, and then come back to your website, that would count as two visits.SessionsA session — which is specific to Google Analytics — is a group of interactions that take place on your website within a given time frame. In other words, a session is the container for the actions a user takes on your site within that time frame. While similar to visits in that one session can involve multiple pageviews, it’s this additional layer of timing that makes sessions unique. By default, sessions in Google Analytics last 30 minutes, but you can adjust this length to last just a few seconds or a few hours at a time. A session ends and a new session starts for a user when either A) there has been 30 minutes of inactivity and the user becomes active again, B) the clock strikes midnight, or C) a user arrives via one campaign, leaves, and then comes back via a different campaign.In contrast to HubSpot’s definition of visits, if a person comes your site, leaves, but returns within 30 minutes, Google Analytics will count it as one session, not two. In addition, if a person is inactive on your site for 30 minutes or more, any future activity on your site is attributed to a new session, even if they’ve never left your site. This is why HubSpot users may see a discrepancy between visits in HubSpot and sessions in Google Analytics. Visitors/UsersA visitor, or a user in Google Analytics, is someone who visits your site. Keep in mind that a single visitor/user can have multiple visits to your site, (or sessions in Google Analytics). In HubSpot’s software, for example, a visitor is tracked by the cookie placed in their browser by the HubSpot tracking code installed on your website. In Google Analytics, how users are calculated is a bit more complicated. If you were to segment your analytics by a particular time frame, for example, the visitors/users count would tell you how many different individual people visited your site during that period of time. New vs. Returning Users/VisitorsA report you’ll find in Google Analytics, this shows the ratio of new to returning users by number of sessions and percentage of sessions. A new user has never been to your site before, whereas a returning user has visited your site in the past. These metrics are great for getting a sense of how well you’re retaining your visitors, and how effective you are in attracting net new visitors at the top of the funnel.Just keep in mind that, because Google Analytics uses cookies to track visitors, the report won’t be 100% accurate in distinguishing new from returning visitors. For example, if a person visits your site via their mobile phone and then on their personal computer, they’ll be counted as new visitors on both occasions (unless you’re using Google’s Universal Analytics capabilities).Got it? Good. 😉 Web Analytics Don’t forget to share this post! AddThis Sharing ButtonsShare to TwitterTwitterShare to FacebookFacebookShare to Email AppEmail AppShare to LinkedInLinkedInShare to MessengerMessengerShare to SlackSlacklast_img read more

How Authoritative Is Your Content? 7 Tools to Help You Find Out

first_img Topics: SEO Resources Originally published Aug 19, 2014 2:00:00 PM, updated February 01 2017 If there’s one thing I love about yoga besides the catnap at the end, it’s that instructors will often remind you that the practice is not a competition. “Don’t let what others are doing affect your practice,” they’ll say. Or, “No one is grading you on your performance.” Lovely sentiments.But not in the content marketing world.Standing out in the elbow-to-elbow class of content marketing practitioners requires more of a competitive spirit than most of us are willing to admit. Perhaps the one saving grace of the content game is that armed with the right tools, you can see how you and your company measure up. By understanding where you stand, you can better optimize content, gauge your authority on specific topics (as well as the authority of your contributors if you have them), track your successes and improve overall content performance.Your analytics are a great place to start to look, but if you want even more metrics to gauge authorship, social, content, and SEO authority, check out the following tools.Content Analysis ToolsAtomic ReachAtomic Reach is a scoring engine that analyzes content based on the quality and relevance in relation to your audience. The central product is the Atomic Engager CMS plugin that enables users to optimize content before it’s published. Available for popular platforms and as a lighter version on their website, the plugin generates an “Atomic Reach” score associated with your audience and grades the content on audience, blog structure, and linguistic elements.Though it won’t help you predict the future, this tool can help you see how well your content is optimized for your audience before you push the publish button. Though the full Atomic product start at $500 per month, the “lite” tool is free to use on their website. Here’s an example of how content displays on Atomic Reach: Influencer Ranking ToolsClearVoiceClearVoice is a tool that gauges author authority with a transparent index of the world’s top content producers categorized by topic. Using Google+ and Twitter authorship markup, the “ClearVoice Score” weighs a writer’s influence by an algorithm that takes into account the domain authority of sites that the writer contributes to, how often the writer publishes, how many sites the author contributes to, and how much the writer’s content is shared on social networks.As a marketer, ClearVoice gives you the power to rate your own performance as a content creator and find authoritative folks in your industry to work with — the ClearVoice Score and Profile are free for public use.BuzzSumoBuzzSumo shows you the most shared links and key influencers for any topic or website. BuzzSumo is useful when creating your content strategy plan because it enables you to perform outreach and competitive research on topics affecting your industry, and see which content on your site is getting the most shares. These results can help you create an editorial calendar around popular subjects. BuzzSumo has a free plan that allows you to perform up to 10 free searches a day and two free content alerts. The “Pro Plan” starts at $99 per month for three users.Social Influence ToolsKloutThough it’s gotten a bad rep as of late, Klout can help you gauge your social influence. Klout is a website and mobile app that uses social media analytics to rank the online influence of its users via the “Klout Score,” which is a value between 1 and 100 — the closer you are to 100, the more influential you are. While it’s not the be-all-end-all metric to get an idea of your success, it can give you a general understanding of how you’re doing on social. Klout recently upped the ante by providing curated content to its users, which is extremely helpful in seeding your own social media feeds with other related content.Klout is free for public use — just make sure you sign in using your Twitter or Facebook account to connect your true social value!KredWhere the algorithm behind the Klout Score can be somewhat murky, Kred provides a more transparent interface of how a person’s “Kred Story” is built.Kred provides two scores: influence and outreach. Your influence is scored on a 1,000-point scale measured by the frequency of how much you are retweeted, replied to, mentioned and followed on Twitter. You also get influence points from your Facebook profile (if connected) calculated from interactions on your wall and those of others with a Kred account. Your outreach score is measured by the your retweets, replies to, and mentions of others on Twitter. Same goes for Facebook if you’re connected. Your outreach score is cumulative and continues to increase with the highest possible current score of 12. Your “Kredenitals” screen summarizes these scores along with most-used hashtags, top communities, interactions, and mentions.Kred is free if you set up a individual account, while Kred for Brands has a cost associated with it.SEO Performance Tools (Note for HubSpot customers: These two tools can give you additional context to the data in your Keywords report.)Moz Open Site ExplorerMoz is a great resource for discovering the SEO prowess of a web page and they offer an few tools to help you improve site performance. Open Site Explorer may be among the most useful for you when it comes to cold, hard data. This database of links data enables you to research which sites are currently linking to yours, uncover links that are linking to old or “broken” pages, research your competitors or peers to see who is linking to them and gain an overall understanding of your domain authority. Authority and link metrics are available for free, however Moz Pro users can see social metrics for rates starting at $99 per month. If you are a part of a company with a smaller marketing budget, stick with the free version of Open Site Explorer.WordStreamWordStream provides tools that help make your search marketing campaigns more organized and ultimately, more profitable. Their suite of Free Keyword Tools give you the power to research the most relevant keywords within your industry including an online SEO keyword suggestion tool, a niche keyword finder, a keyword list grouper tool, and a negative keyword finder. These tools help you manage your paid and organic search initiatives with a better understanding of the best (and worst) keywords within your vertical. The Free Keyword Tool is free for users: You get 10 free keyword searches up-front, then one free keyword search per day going forward.While many of these tools overlap on the metrics they provide, they can each give you an awareness of the various components that affect your effectiveness as a digital marketing practitioner. Find the tools that work for you and commit to using them over an extended period of time so you can understand the nuances of each while tracking your success. And if you need a refresher on the overall importance of online authority and how SEO and social media work together to improve your performance as a marketer, you can download the iAcquire and HubSpot’s ebook, Intersection of SEO and Social Media. Don’t forget to share this post! 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How to Be a More Curious Person: 7 Tips for Becoming a Lifelong Learner

first_imgThe other day, I was in the grocery store. I overheard a cart-bound little kid asking his mom question after question. “Mom, why do they put so many apples out at once?” he asked as they browsed the produce section. “Mom, what’s Ocean Spray?” he speculated from one aisle over.His enthusiasm and investigative demeanor got me thinking about my own level of curiosity. After all, in today’s fast-paced, innovation-heavy world, what we know today might not be valuable to us in a few weeks, months, or years. And if we want to remain agile, we need to continue to seek out and explore new information, new mediums, and new opportunities.We need to be more curious.While it may seem like an innate desire, there’s actually a lot you can do to nurture and increase your own curiosity. From asking more questions to embracing the unexpected, the following tips are designed to help you become more curious.How to Be a More Curious Person: 7 Tips for Becoming a Lifelong Learner1) Ask every question.Try as we might, it’s impossible to have all the answers, all the time. And while it’s easy to default to assumptions when faced with a challenging unknown, it’s better to work through knowledge gaps by asking questions. When we ask questions, we’re creating an opportunity to discover new, useful information that can be used to challenge our existing approach, expand our vision, and spark fresh ideas. Questioning allows us to become more insightful.”People who get insights see something that’s a little bit off, and instead of ignoring it, they’re curious about it. Curiosity keeps our mind engaged to work out the implications,” explains cognitive psychologist Gary Klein.Don’t know what questions to ask? The folks at HopeLab, a research and development organization, created a series of questions for curious leaders. Designed to help people remain both present and open, these prompts promote the importance of curiosity when assessing a situation. 2) Consume content that’s outside of your comfort zone.I never realized how much there was to learn about coffee pots until mine stopped working one morning. It’s funny the way that works, isn’t it? When we don’t have any initial knowledge about something, we’re not really curious about it … that is, until we have to be. Back in 1994, George Loewenstein, a professor of economics and psychology at Carnegie Mellon University, suggested that curiosity does, in fact, require some initial knowledge. In other words, the more we know, the more we want to know. So if you’re looking to become a bit more curious, you may want to try tapping into some subject matter you don’t know much about. Whether you focus on productivity habits, Egyptian pyramids, beer brewing or something entirely different is up to you. The important part is that the content is unfamiliar — basic research will spur you to want to know more.3) Listen without judgment.”Curious people are often considered good listeners and conversationalists,” explains Ben Dean, Ph.D. in a newsletter for the University of Pennsylvania. By suspending judgment, you’re ultimately allowing yourself to be more receptive to what someone is saying. You’re focusing less on what you’re going to say next, and more on the words and information they’re choosing to tell you — or not tell you.So next time you’re having a conversation with someone, just listen. When you take the time to truly absorb what they are saying, it’ll be easier for you to formulate questions, warm up to new perspectives, and learn something new that you may have missed otherwise. 4) Embrace the unexpected. Many people find themselves plagued by their own doubts, which causes them to miss out on new places, flavors, people, and experiences. But often times, doing something unexpected can trigger a chain of reactions — both positive and negative — that you can learn from and formulate questions based on. When you’re busy worrying about the “what ifs,” you’re shutting yourself off from these types of unexpected opportunities. So go ahead, book that plane ticket. Go on that blind date. Say yes to the “chef’s special” at a restaurant — even if you’re not quite sure how to pronounce it. Research reveals that surprise can actually drive our motivation to learn, so embrace the unexpected and celebrate whatever the outcome. 5) Try not to dwell on the past. When you spend all your time worrying about the past, you don’t give yourself a chance to be curious about the future. So rather than worrying about what you could have, would have, or should have changed about your last assignment, try to focus on strategies for blowing your next project out of the water. “The problem for many adults is that we stop being curious about new experiences and are instead focused on understanding what we’ve already been through,” explains David Klow, founder of Skylight Counseling Center.What new opportunities are out there for you to explore? Is there anyone in your network who can help you get better at XYZ? What conferences can you attend to expand your understanding of the industry you work in? Focus on what’s to come, not what’s already happened. 6) Gamify learning.  If you tend to view learning as dull or tedious, you’ll probably find that training yourself to be more curious isn’t easy. As you would imagine, this is a problem that many teachers are forced to overcome when dealing with unmotivated students. To inspire curiosity and excitement, many teachers have turned to gamification in the classroom. By gamifying lessons, they’re able to regain the attention and interest of their students. Why does this approach work? Games make us “more curious, more determined, and more optimistic,” explains game designer and author Jane McGonigal. According to her research, games allow us to try — and try again — without having to worry about failure, which helps to motivate us to keep going.If you’re having trouble staying curious about a subject, try turning your research into a game. Check out Quizlet’s game mode to brush up on subjects by completing challenges such speed tests and drag-and-drop matching games. 7) Change your perspective on a situation.Rather than rest on your laurels when it comes to making decisions or planning projects, allow yourself to view the situation from the perspective of a stranger — or better yet, your buyer persona. What would they suggest? What concerns would they have? What challenges do they face? Then, look at the situation through the eyes of your competitor, your boss, an industry outsider, etc. and continue the exercise.”When we are curious about others and talk to people outside our usual social circle, we become better able to understand those with lives, experiences, and worldviews different than our own,” explains Emily Campbell, research assistant at UC Berkeley’s Greater Good Science Center. More curiosity and empathy is a good thing — it can go a long way to making you a better marketer (and human). What are your favorite tips for thinking more curiously? Share them with us in the comments section below. Don’t forget to share this post! AddThis Sharing ButtonsShare to TwitterTwitterShare to FacebookFacebookShare to Email AppEmail AppShare to LinkedInLinkedInShare to MessengerMessengerShare to SlackSlack Topics: Originally published Jan 7, 2016 6:00:00 AM, updated July 28 2017 Curiousitylast_img read more

Jamaica to Host ECPA Energy Summit in 2019

first_img Extending appreciation to President of Chile, Michelle Bachelet and the Minister of Energy, Hon. Andres Rebelledo and his team, in partnership with the OAS, for guiding ECPA over the past two years, she noted that Chile has handed Jamaica the baton with a running start. She expressed confidence that Jamaica will also provide strong leadership. Ambassador to the United States and Permanent Representative to the Organization of American States (OAS), Her Excellency Audrey Marks, made the announcement as she addressed the opening plenary of the 3rd Ministerial Meeting of ECPA, at Viña del Mar, just outside Santiago, Chile, on September 7. Jamaica is to host the 5th Ministerial Meeting of the Energy and Climate Partnership of the Americas (ECPA), scheduled to be held in 2019. Story Highlights Jamaica is to host the 5th Ministerial Meeting of the Energy and Climate Partnership of the Americas (ECPA), scheduled to be held in 2019.Ambassador to the United States and Permanent Representative to the Organization of American States (OAS), Her Excellency Audrey Marks, made the announcement as she addressed the opening plenary of the 3rd Ministerial Meeting of ECPA, at Viña del Mar, just outside Santiago, Chile, on September 7.The Ambassador spoke of the continued commitment of the Government towards energy diversification and access, and reduced cost to the population.She said Jamaica looks forward to the next two years of leading the work of the ECPA in partnership with the team at the Development Secretariat of the OAS.“The region is rich in natural resources and we will seek to optimize this potential through various activities that will make the Americas a leader in the new energy paradigm,” the Ambassador said.Extending appreciation to President of Chile, Michelle Bachelet and the Minister of Energy, Hon. Andres Rebelledo and his team, in partnership with the OAS, for guiding ECPA over the past two years, she noted that Chile has handed Jamaica the baton with a running start. She expressed confidence that Jamaica will also provide strong leadership.“I am very heartened by the expressions of support from the partners of this initiative at the United States Department of Energy and Department of State as well as the Inter-American Development Bank (IDB) and World Bank delegations,” the Ambassador said.Also addressing the meeting were President Bachelet and the Secretary General of the OAS, Ambassador Luis Almagro.President Bachelet highlighted the necessity of energy access and her country’s commitment towards more sustainable energy, and supporting the guiding principles and Action Plan of the ECPA.During the opening ceremony, representatives from several countries made presentations in support of the seven pillars of the ECPA.The seven pillars include: promoting renewable energy; improving energy efficiency; making more efficient use of fossil fuels; creating energy infrastructure; reducing energy poverty; promoting regional energy integration; and developing energy research and innovation.Its Mission is to promote regional energy cooperation through different strategies and actions to achieve a cleaner, safer, efficient, modern and fair energy deployment, while the Vision is one of shared leadership in the implementation of energy initiatives and the exchange of experiences within the countries of the Americas, in support of energy development objectives.The ECPA is supported by a Technical Coordination Unit led by the Department of Sustainable Development of the OAS.last_img read more

ADNOC Cuts Fuel Consumption CO2 Emissions by 23 Pct

first_imgzoomIllustration. Image Courtesy: Pixabay under CC0 Creative Commons license ADNOC Logistics & Services (ADNOC L&S), the marine and logistics subsidiary of the Abu Dhabi National Oil Company (ADNOC), said that it has reduced fuel consumption and carbon emissions by 23% across a fleet of 27 vessels.The fuel consumption and carbon emission reduction efforts form part of ADNOC’s ‘Al Daffah Energy Efficiency Project, which relied heavily on digitization to improve energy performance, aid in route planning, improve fuel monitoring and optimize engine speed.The installation of more efficient propellers and high-performance hull coating have also been among vessel upgrades implemented by the company to improve vessel thrust.The company added that it took advantage of favorable market rates to charter an additional LNG vessel, ensuring that slow steaming could be adopted across the fleet, without affecting delivery schedules.ADNOC said that to date, the accumulated fuel savings from the ‘Al Daffah Energy Efficiency Project’ are 325,000 metric tons of heavy fuel oil – which is equivalent to the load of a VLCC tanker.“Together with our industry partners and classification societies, we have constantly re-assessed, challenged and developed our capabilities to deliver more for less, in a challenging market. As the results show, the level of dedication towards achieving our ambitious targets has been unfaltering throughout this project,” Captain Mohammed Al-Alli, Senior Vice President, Ship Management at ADNOC L&S, said.ADNOC L&S, which owns and operates 27 shipping vessels and a total fleet of 122 shipping and offshore vessels, claims to have reduced its CO2 emissions by 1 million tons since 2013.last_img read more

Supreme Court Rejects Kingfisher Airlines Plea for Legal Representation

first_imgThe Supreme Court on Tuesday disapproved a plea from debt-hit Kingfisher Airlines to be allowed legal representation before the grievance redressal committee set up by United Bank of India, after the bank declared it as wilful defaulter on Monday.The company defended itself by stating that the bank took the step though the airline’s leave petition against the Calcutta High Court order is still pending in the Supreme Court. But the Supreme Court replied that the plea is fructuous now.”Now that you have been declared a defaulter, you can challenge it in any court. Move a high court for an appropriate order. Your grievance has been, that Grievance Redressal Committee (GRC) should not decide the matter but they had already decided it, therefore your plea is in fructuous”,” said a bench headed by Justice Anil R Dave, while declining to admit KFA’s petition.Kingfisher Airlines claimed that UBI had rapidly declared it as a wilful defaulter, without following norms of justice, reported the Financial Express.Along with Vijay Mallya, the Kolkata-based bank has also tagged three other directors of Kingfisher Airlines – AK Ganguly, Subhash R Gupte and R Nedungadi – on the list.The Grievance Redressal Committee (GRC) meeting was planned on Monday, after the Calcutta High Court allowed the bank to initiate the process of declaring the company as wilful defaulter last week. The GRC order forced the company to attend the meeting but no one was present from the company. This forced the bank to take this step.Instead of attending the meeting, KFA sent a letter through their lawyer, stating that the company had filed a Public Interest Litigation (PIL) in the Supreme Court.UBI has become the first bank to declare Mallya as wilful defaulter.Following the decisions by United Bank of India, other lenders such as the State Bank of India, IDBI bank and Punjab National Bank have also started the process of tagging KFA and Mallya’s group as wilful defaulter.last_img read more

Britains EU exit campaign backed by 250 business leaders

first_imgThe campaign for Britain to leave the EU has been backed by 250 business leaders, including a former chief executive of HSBC, the Vote Leave group said Saturday, hoping to counter the view UK businesses back staying in the bloc.The camps arguing for and against Britain staying in the European Union ahead of a June 23 referendum on British membership have both made the economic impact of a “Brexit” a cornerstone of their campaigns.Last month, the bosses at more than a third of Britain’s biggest companies, including major oil companies Shell RDSA.L and BP, and its largest telecoms group BT, said leaving the EU would put jobs and investment at risk.Vote Leave, one of the groups supporting a British exit, unveiled Saturday its own list of backers, including Michael Geoghegan, former chief executive of HSBC Group and Tim Martin, the boss of pubs group JD Wetherspoon.”With our growing list of business supporters, Vote Leave will make that case that whilst the EU might be good for big multinationals, for smaller businesses it acts as a job destruction regulatory machine,” Matthew Elliott, chief executive of Vote Leave, said.However, the Sunday Times newspaper reported two of the well-known names on the list — John Caudwell, founder of Phones4U, and David Ross, the co-founder of Carphone Warehouse — had not signed up.”You have to question how this list has been compiled,” the paper quoted a spokesman for Caudwell as saying.Vote Leave also said it was forming a Business Council to argue that EU membership was holding back business.That group will be headed by John Longworth, who quit as director general of the British Chambers of Commerce (BCC) lobbying group after he spoke out in favour of leaving the EU, accusing Prime Minister David Cameron of trying to scare voters into backing his case to stay in the bloc.Meanwhile, the Times newspaper reported that hedge funds were planning to use exit polls to make big profits on the day of the referendum.Under electoral law, it is illegal to publish the results of such polls while people are still voting, but a private poll could allow traders to exploit moves in the currency market, with the sterling expected to rise sharply against the dollar on the back of an “In” vote but decline if Britons vote for an exit.The sterling fell to multi-year lows this week on a perceived rise in the chances of an EU exit, and on companies and fund investors hedging against it, though the currency later steadied.”There is a lot of interest around Brexit, particularly from the big U.S. funds,” one unnamed broker told the Times.The odds of a Brexit narrowed Tuesday as the Brussels bombings were seen boosting the “Out” campaign.last_img read more

Bangladesh fifth largest wheat importer

first_imgProthom Alo File PhotoBangladesh has emerged as the fifth largest wheat importer in the world, with its import of the grain increasing by 36 per cent over the past five years, according to a report of the United Nation’s Food and Agriculture Organisation (FAO).Among the fastest growing importing countries, Bangladesh imported 5.5 million tonnes of wheat in the last fiscal. FAO predicts this is likely to increase to 6 million tonnes in the current fiscal year.Even just five years ago, Bangladesh was not among the top 10 wheat importing countries of the world.Changing in food habits and increased health awareness has made people to turn more to wheat, consequently raising the demand of the grain, said former director of the Institute of Nutrition and Food Science Nazma Shahin told Prothom Alo.“People nowadays eat less rice as wheat contains more protein and provides more energy. On the other hand, rice contains starch that may cause diabetes. Also, wheat is cheaper than rice,” Shahin explained.The top four countries ahead of Bangladesh in importing wheat are Egypt, Indonesia, Algeria and Brazil. These countries imported 507 million tonnes wheat in the previous fiscal year.The top exporting countries are the United States, Russia, Canada, Australia and Ukraine.According to the FAO report, overall wheat production increased by 20 per cent over the past five years.The production increased from 900,000 tonnes to 1.2 million tonnes in 10 years.Usually the wheat available in the local market is mixed with corn. Corn production has also increased to 39 million tonnes last year. Most of the corn is mixed with wheat to prepare poultry and fish feed.Meanwhile, government statistics show, the risk of diabetes is increasing among the adults of the country. The government’s 2006 survey on non-communicable diseases (Steps 2006) showed about 5.5 per cent of adults were suffering from diabetes while another similar survey (steps 2018) showed 6.4 per cent of adults suffer from this disease in Bangladesh. That means more than 7.6 million people are suffering from diabetes.Physicians advise eating roti (flat bread) instead of rice to control blood sugar levels. Apart from this, many people eat rice once a day and bread in the two meals other meals, out of health awareness. There is thus an increased consumption of roti, bread and wheat products in the country.With growing urbanisation and increased per capita income, the consumption of roti, fish, meat and dairy products will increase, says country representative Akhter Ahmed of Bangladesh Policy Research and Strategy Support Program (IFPRI).“A large number of working people eat out nowadays in Bangladesh. This is positive, but the quality of food in the outside eateries should be ensured,” he added.*This piece originally published in Prothom Alo print edition has been rewritten in English by Farjana Liakatlast_img read more

Bangladeshi killed by BSF BGB

first_img.A Bangladeshi national was shot dead by Indian Border Security Force (BSF) at Burimari border in Patgram upazila of Lalmonirhat on Sunday, reports UNB.The deceased is Arshadul Haque, 30, son of Ehsanul Haque of Islampur village of the upazila.Commanding officer of Border Guards Bangladesh (BGB) Rangpur-61 battalion lieutenant colonel Shariful Islam said when Arshadul went to 840/3 Sub pillar of the border to bring cattle, a patrol team of BSF fired gunshot on him, leaving him dead.BGB has sent a letter to BSF for holding a flag meeting in this regard, he added.last_img

This Ceasefire Weekend House Music Heals

first_imgBy Sean Yoes, AFRO Baltimore Editor, syoes@afro.comSo far in 2019 Baltimore has been averaging almost a murder a day, 26 homicides as of Jan. 26.There have been six days with multiple homicides. There was a homicide every day from Jan. 8 – Jan. 13. We’ve gotten off to a bad start after registering moderately lower crime rates across the board in 2018.It seems Ceasefire Weekend is coming right on time, as usual.“So, the movement itself is based on the idea that Baltimore can collectively put a pause on violence, if we decide as a city, as a community we can do that. We can give one another at least 72 hours,” said Letrice Gant, a co-founder of Baltimore Ceasefire 365, during a recent conversation I had with her at Impact Hub on North Ave.“The other part of that, the other component we try to stress to people is this idea about celebrating life,” Gant added. “So, one of the things we encourage people to do during Ceasefire Weekend is to plan what we call life affirming events. Life affirming events can be something as intimate as you sitting down with family members and having a conversation about the context of violence and how it impacts you directly.”The essence of Baltimore Ceasefire and perhaps the main reason the Ceasefire Weekends have been so successful (every Ceasefire weekend has seen a significant decrease in homicides and shootings), is that it is grass roots and  community-driven. Ceasefire is not some lumbering entity hovering over the community, handing down edicts to end violence, which the residents of the mostly Black, mostly poor communities most affected by violence would promptly ignore. Ceasefire is of, from and for those communities most impacted by murder and mayhem and the residents of those communities are most important in the battle to change the city’s injurious trajectory.From the beginning (Aug. 21), I’ve thrown my personal and professional support behind the Ceasefire movement led by Erricka Bridgeford, Gant, Ogun Gordy, Jakia Jason, Michelle Herring and Darnyle Wharton.But, this Ceasefire Weekend I’m in the game for real–along with many others creating space for peace and healing.Me and my partner Tiffany Ginyard, (the AFRO’s new managing editor), who happens to be one of the smartest, most talented young people I know, are producing “House Music Heals,” a “life affirming event” at the burgeoning Impact Hub on North Ave.I may not be totally objective here but, House Music Heals is unique in the sense we are convening a community conversation about the impact of violence on our young people, as well as a traditional Baltimore House Party. Both elements of our forum lend themselves to healing.Our conversation about the impact of ubiquitous violence (more than 50 percent of 2018 homicides were people aged 13-30) features five dynamic young leaders. Lauren Bell, a 10th grade a multi-talented artist and writer; Deleciea Greene and Keyma Flight, members of the nationally respected Dewmore Baltimore Youth Poetry Team; Khaneef Goode, a West Baltimore community organizer and Ras Tahuti Missouri, another powerhouse spoken word artist.I first wrote about Missouri in July 2016, when he reflected on the death of murdered West Baltimore rap artist “Lor Scoota” (Tyriece Watson). “He was trying to get away from the street life, he was trying to get away from all of that,” Missouri, who was 15 at the time told me back in 2016. “That was his ticket out of the city…rapping about what he lived…and it’s sad that the reality and what he lived turned around to be his downfall.”And it is a divine twist of fate that we are also facilitating a House Music set across the street from the mythic Odell’s nightclub. Although Odell’s has been shuttered for decades, the creative energy is crackling once again on North Avenue. Our House Music party features two phenomenal DJs: Henry Da Man (Henry Featherstone) and Sunny T (Monica Taylor). I’ve known Taylor since we were both in Middle School and although she’s always had a deep love of music, she just started DJ’ing a few years ago. Featherstone, who I refer to as “the Professa,” is one of the most knowledgeable DJ’s in Baltimore, a bastion of world class House Music talent.Sean Yoes (Courtesy Photo)I wrote about “The Healing Power of House Music” in October 2015, when I referenced the legendary “Deep Sugar” House party, during a special set by Ultra Nate and Lisa Moody shortly after the Uprising of April 2015.I wrote that Deep Sugar was a spiritual salve for many of the city’s disenfranchised; people of color, the LGBT community and many others.We need that healing power every day.Sean Yoes is the AFRO’s Baltimore editor and the author of Baltimore After Freddie Gray: Real Stories From One of America’s Great Imperiled Cities.last_img read more

Apple Announces Education Event in Chicago

first_imgHowever, more recently, Google has been increasingly successful with its Chromebooks, which are generally cheaper and easier to maintain by school staff. Apple’s struggles in the education market were on full display when the Los Angeles school district backed out of a plan to buy iPads for all of its students in 2015, ultimately reaching settlements with both Apple and education software provider Pearson to refund some of its expenses. Apple has invited media to an education-focused event in Chicago, which will be held on March 27th at a local high school. The invite, which was sent out Friday morning, featured the slogan “let’s take a field trip.”In typical Apple fashion, the company didn’t reveal anything about the news it intends to announce at the event, instead simply stating that it was about “creative new ideas for teachers and students.”There have recently been rumors that Apple could release a cheaper Macbook, as well as possibly a new entry-level iPad to complement its more expensive iPad Pro line. Especially the latter could make an interesting product for schools, and possibly help the company catch up with competitors.Some years ago, Apple was very successful at bringing its iMac to schools across the country, which helped the company to effectively build a pipeline: Students who grew up with Macs at school were more likely to buy them later in life as well. ×Actors Reveal Their Favorite Disney PrincessesSeveral actors, like Daisy Ridley, Awkwafina, Jeff Goldblum and Gina Rodriguez, reveal their favorite Disney princesses. Rapunzel, Mulan, Ariel,Tiana, Sleeping Beauty and Jasmine all got some love from the Disney stars.More VideosVolume 0%Press shift question mark to access a list of keyboard shortcutsKeyboard Shortcutsplay/pauseincrease volumedecrease volumeseek forwardsseek backwardstoggle captionstoggle fullscreenmute/unmuteseek to %SPACE↑↓→←cfm0-9Next UpJennifer Lopez Shares How She Became a Mogul04:350.5x1x1.25×1.5x2xLive00:0002:1502:15center_img Popular on Variety last_img read more

Face pass is latest security system for NEC laptops

first_img Citation: ‘Face pass’ is latest security system for NEC laptops (2007, September 4) retrieved 18 August 2019 from NEC´s LaVie series will offer “face pass” security. The LaVie C and LaVie L series will both include the new facial recognition software, which enables only a programmed user to log on to the computer.NEC’s software, called “NeoFace,” is a biometric system that uses a combination of eye zone extraction and facial recognition to identify the computer’s user. To program the system, a user sets up a profile with three photographs of their face. Then when a user tries to log on, an integrated 2.0 megapixel camera scans their facial characteristics.The NeoFace system then uses a matching procedure to determine the identity of the user. NEC says that the system performs accurate matching even when people wear glasses and hats, have different haircuts or facial hair, and show different facial expressions. The ability to distinguish between identical twins is still speculative.“NeoFace uses a technology called ‘adaptive region mixed matching,’ which focuses on ‘segment regions’ with a high degree of similarity for matching,” explained Atsushi Sato, a head researcher at NEC. “Other makers’ products make judgments based on a number of combined characteristics, such as the distance between the eyes and the nose, or the nose and the mouth. But this creates a problem, because if even one of these segments is missing, the accuracy drops dramatically. “In contrast, NeoFace divides the input image and the registered image into small segments, and focused only on the segments that are highly similar,” he continued. “This enables the system to achieve higher authentication accuracy than out competitors’ products, even if a part of the subject’s face is hidden, for example by a mask or sunglasses.”NEC originally developed NeoFace for security applications, such as border control, prison management and corporate security, to eliminate the need for fingerprints. In July 2007, NEC announced the first automated border control system to use facial recognition technology that can identify people inside their cars. At checkpoints on the Hong Kong-Shenzhen border, the system reads a car’s license plate, and compares the driver’s face with the registered driver’s micro-chipped ID.“The main advantage to face authentication is convenience,” said Kazuyori Miyaoka of NEC’s Business Promotion Department. “A number of other authentication methods are currently in the research stage, for example using the shape of the ears or the patterns of blood vessels on the back of the hand, as well as a person’s walk, smell, DNA or keystroke habits when using a PC keyboard.”As for the consumer laptops, both the LaVie C and LaVie L series are expected to be available in the Japanese market in late September. Besides the face pass system, the laptops will have mostly standard features: 15.4-inch displays, Core 2 Duo processors on the top-end LaVie C model or the option of either Core 2 Duo or Celeron processors on the LaVie L model. Blu-ray is also available for the high-end model. The price is expected to range from ¥150,000 to ¥310,000 (or about $1,300 to $2,675).Some of the above information was adapted from NEC. NEC has launched two new series of laptops with a unique security feature called “face pass” — or, in Japanese, “kao pass.” This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.last_img read more

EcoFriendly Surveillance NEC Develops Enhancement That Uses Fluorescent Light Tubes

first_imgNEC spy camera – Credit: NEC & Digital World Tokyo Minuscule microbes wield enormous power over the Great Lakes, but many species remain a mystery Citation: Eco-Friendly Surveillance: NEC Develops Enhancement That Uses Fluorescent Light Tubes (2007, December 3) retrieved 18 August 2019 from NEC has developed a wireless security camera that utilizes energy from fluorescent light tubes. Workplace employee surveillance cameras can be neatly tucked away in the false ceilings along side the fluorescent tubes. The surveillance camera is tethered to the fluorescent light by a single wire with a ring-like adapter at one end. As reported by Digital World Tokyo, the camera is activated when the lights are turned on. The act of flipping the light switch activates the electricity generated by the Sharp-created technology located in the ring through electromagnetic induction. The magnetic field created by the AC source in the fluorescent light tubes is the energy source the technology development utilizes. The magnetic field frequency of 45 to 100 kHz can be used by the ring to generate enough electricity,(120 mW), to power up the camera. The camera is a standard-VGA resolution unit that takes pictures every ten seconds. The images are transferred to a PC utilizing an ordinary WiFi chip that draws power from the fluorescent light source. NEC is a leader in surveillance and security solutions for the 21st Century. NEC envisions the use of the energy saving technology will be useful for merchants in determining on-site marketing trends. Other uses include hotel surveillance for security purposes. In the work place, the unobtrusive surveillance system employs green technology and may entitle purchases to be offset by applicable rebates and credits. NEC is focused on promoting environmentally conscious technologies and enhancements for current devices. NEC is a member of the “Team Minus 6% Project,” whose mission it is to reduce energy consumption by six percent. NEC is currently involved in the development of solar power for PCs, recycling and the utilization of energy saving fluorescent light tubes as well as other alternative Eco-friendly energy sources. Copyright 2007 Mary Anne Simpson & All rights reserved. Web Sites and Bloggers may provide the introductory paragraph and a link to the story, but may not copy, redistribute, rewrite or publish the story in whole or in part without written permission of the author or publisher. This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only. Explore further NEC has developed an Eco-Friendly adaptation that allows surveillance cameras to utilize energy from fluorescent light tubes. The surveillance system can be tucked into the false ceiling next to the fluorescent lights.last_img read more

Small is beautiful Viewing hydrogen atoms with neutron protein crystallography

first_img Research Professor Julian C.-H. Chen discussed the group’s research with, first summarizing the main challenges they faced in overcoming the limitations of only X-ray crystallography, visualizing hydrogen atoms a three-dimensional context, determining protonation states of active site residues in enzymes, and establishing the identities and orientation of solvent molecules. “The strength of neutron crystallography lies in its ability to locate hydrogen atoms in macromolecular structures. Hydrogen accounts for one-half of the atoms in any given protein, and help perform all sorts of tasks, from hydrogen bonding to catalysis,” Chen explains. “For X-ray crystallography, locating hydrogen atoms is especially difficult as they possess just one electron, and X-rays are weakly scattered by hydrogen atoms compared to other elements in proteins such as carbon, oxygen, nitrogen, and sulfur.”Furthermore, hydrogen atoms in protein structures can be quite mobile. “You’re lucky to be able to see them even in the highest resolution structures,” Chen continues. “Neutrons, on the other hand, have the nifty property that deuterium, which can be introduced into reactive sites on the protein through hydrogen/deuterium exchange, scatters neutrons so they are easily located and readily visible in nuclear density maps, even at moderate resolution. So this makes it relatively straightforward to determine protonation states of residues. Similarly, for solvent molecules, D2O, or heavy water, can be introduced into the crystal – and these molecules have an easily identifiable boomerang shape in the nuclear density maps, which makes it possible to orient them.”On addressing these challenges, Chen adds, “We reasoned that if we had the combination of ultrahigh resolution and neutron diffraction data on hydrogen/deuterium-exchanged crystals, we should be able to describe how individual deuterium atoms vibrate – and not only that, but also describe their anisotropic vibrational motion.” (An anisotropic material exhibits different values of a property in different crystallographic directions.) “So that’s exactly what we did for a number of selected deuterium atoms within crambin – a protein that forms the best-ordered macromolecular crystals known. We surmised that crambin crystals would diffract neutrons to ultra-high resolution as well.” (—Creating 3D visualizations of hydrogen atoms in proteins is especially challenging, often requiring their locations to be inferred from those of nearby carbon, nitrogen, oxygen or sulfur atoms stored in protein structure databases. These locations are based on atomic positions in databases of previously solved structures, general chemical knowledge, quantum mechanical calculations, or potential hydrogen bonding interactions. While X-ray crystallography can pinpoint hydrogen atom locations at ultrahigh resolution, in practice only a few such positions are experimentally determined. Recently, however, scientists at the University of Toledo, Los Alamos National Laboratory and Oak Ridge National Laboratory used neutron crystallography – a technique that even at lower resolutions can locate individual hydrogen atoms by leveraging scattering properties of the hydrogen isotope deuterium. More information: Direct observation of hydrogen atom dynamics and interactions by ultrahigh resolution neutron protein crystallography, PNAS September 4, 2012, doi:10.1073/pnas.1208341109 Explore further Structure of enzyme against chemical warfare agents determined Copyright 2012 All rights reserved. This material may not be published, broadcast, rewritten or redistributed in whole or part without the express written permission of This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only. Journal information: Proceedings of the National Academy of Sciences As Chen points out, crambin is a small protein with a limited number of deuterium atoms. “Especially with the new neutron crystallography instruments under construction or being commissioned, new datasets of similar or higher resolution should be possible with crambin as well as other proteins. We’re of course staying updated on these developments.” Chen adds that some of their findings pertaining to hydrogen/deuterium exchange can also be addressed by other physical methods.Chen also comments on their key findings provide the ability to inventory all hydrogen bonding interactions within the protein and ascertain the categories and suggest strengths of hydrogen bonds in the system; demonstrate the power of ultrahigh resolution neutron diffraction to resolve ambiguous hydrogen bonding networks; and reveal unconventional structural features and interatomic interactions. “You generally only see the oxygen of the water in X-ray structures, keep in mind that the two hydrogens scatter X-rays weakly and are generally invisible.” This means that the hydrogen bonding networks can be ambiguous. “But with neutrons,” Chen continues, “since you get strong scattering from the deuteriums of the D2O, you can orient the hydrogens and figure out the network of hydrogen bond donors and acceptors.”Moreover, says Chen, the researchers also saw some instances of unusual hydrogen/deuterium exchange patterns. “One residue in particular appeared to have hydrogen/deuterium exchange on one of the hydrogens bonded to the alpha carbon. This could be evidence of C-H. . .O hydrogen bonds, which are rather elusive and difficult to observe experimentally although nearly 30 years of mostly computational work have been done on these hydrogen bonds.”In terms of other research and downstream technology might benefit from your findings, Chen stresses that their study demonstrates what can be done with neutron crystallography. “I think that this, and future structures, will give the humble hydrogen atom its due,” Chen concludes. “This will hopefully work its way into computational algorithms that address protein-ligand and protein-drug interactions. This work may also lead in the long run to more accurate, higher quality macromolecular structures.” (A) Structure of crambin, with main chain amide hydrogen/deuterium (H/D) exchange pattern ranging from blue (unexchanged) to red (fully exchanged). (B) Side chains of residues R10, N14, N46, and T2 are depicted with anisotropic ellipsoids. D atoms are shown in green, with hydrogen bonds indicated. Copyright © PNAS, doi:10.1073/pnas.1208341109 Citation: Small is beautiful: Viewing hydrogen atoms with neutron protein crystallography (2012, September 20) retrieved 18 August 2019 from read more